Taiwan Seed Improvement and Propagation Station,MOA
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The Establishment of high through-put Sequencing technique to detect potato virus

Fig 1. We used tissue culture techniques to evaluate PVX from potato, and then used NGS techniques (the small RNA profiles) to conform that the potato material not only PVX-free but also virus-free.
▲Fig 1. We used tissue culture techniques to evaluate PVX from potato,
and then used NGS techniques (the small RNA profiles) to conform that
the potato material not only PVX-free but also virus-free.

Fig 2. The small RNA NGS profile of potato sample No.2 showed that ToCV was detected in dry-lab techniques. We used RT-PCR and sequencing such wet-lab methods to confirm these results.
▲Fig 2. The small RNA NGS profile of potato sample No.2 showed that
ToCV was detected in dry-lab techniques. We used RT-PCR and
sequencing such wet-lab methods to confirm these results.



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