|Home > Achievement > 2019 Research Project List > Establishment of inspection and monitoring system for new era genetically modified organisms|
▲Fig 1. RuBisCO small subunit (rbcS) terminator (tE9) is frequently used transgenic element in RoundupReady2 herbicide resistant GM soybean. We cloned tE9 element derived from imported feed soybean and established reliable reference plasmid for GM detection.
▲Fig 2. The G1H1 is new generation GM rice event with insect and herbicide resistance. We constructed a positive control plastid for detection (ProGene620 OsG6H1) based on publicly available sequence by artificial synthesis method. The detection primer G6H1-F / R was selected for event detection. Furthermore, the testing results indicated using 20 ~ 2 pg DNA template for PCR, and the detection limit is 20 ~ 2 fg.
▲Fig 3. To evaluate GM crops detection sensitivity and efficiency, we used SYBR Green stain cope with real-time PCR assay for GM crops screening.