2026 Research Project List
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Establishment of Molecular Techniques for Rose Variety Identification

Taiwan's plant variety protection (PVP) system is established under the Plant Variety and Plant Seed Act and adopts the criteria of Distinctness, Uniformity, and Stability (DUS) as the primary basis for variety rights examination. However, as the genetic background of commercial cultivars has become increasingly narrow, conventional field-based morphological evaluations are not only time-consuming but also susceptible to environmental influences, making them progressively less capable of meeting the demands of variety identification and infringement determination.

In contrast, molecular markers offer high stability, excellent reproducibility, and are largely unaffected by environmental conditions. Consequently, they have been increasingly applied to plant variety identification and the protection of plant breeders' rights. Roses are among the most important ornamental crops in Taiwan, and recent plant variety rights disputes have highlighted the need to establish objective and scientifically reliable methods for variety identification.

In this study, rose (Rosa spp.) cultivars protected under Taiwan's plant variety rights system were collected as research materials. A DNA extraction protocol suitable for roses has also been preliminarily established to serve as the foundation for subsequent molecular analyses. Future work will focus on developing simple sequence repeat (SSR) markers and establishing a molecular fingerprint database for rose cultivars. These tools will be applied to variety identification, genetic relationship analysis, and plant variety rights infringement determination, thereby providing a scientific basis for infringement assessment and serving as a valuable reference for future third-party identification and the protection of plant variety rights.

Figure 1. Preliminary establishment of a genomic DNA extraction protocol for rose. The extracted DNA was validated by housekeeping gene amplification and showed stable amplification performance, providing a foundation for subsequent molecular marker analyses.
▲Figure 1. Preliminary establishment of a genomic DNA extraction protocol for rose. The extracted DNA was validated by housekeeping gene amplification and showed stable amplification performance, providing a foundation for subsequent molecular marker analyses.