2025 Research Project List
This study established anther culture induction conditions for three Brassicaceae cultivars and successfully obtained callus on C1 and C3 media. However, the overall regeneration efficiency remained low, indicating the need to further optimize callus differentiation conditions. Regenerated shoots transferred to MS medium continued to grow normally, with preliminary observations showing that the kale cultivar M exhibited the highest regeneration capacity. For three local green bamboo lines, stable shoot proliferation and rooting protocols were developed, and the modified 167-3 medium significantly enhanced the proliferation rates of the 'Dapang' and 'Hongyu' lines. Long-term subculture-based flowering induction trials revealed a gradual decline in proliferation rates, with no significant differences in final plant growth performance among treatments and no successful floral induction. Further refinement of medium formulations and induction methods will be required to enhance flowering responsiveness.
 ▲Fig 1. Regenerated plants of cruciferous crops induced by anther culture |
 ▲Fig 2. Growth of tissue-cultured seedlings of local green bamboo lines after rooting culture. |
