2025 Research Project List
Second-generation (G2) individual plants EG195, EG203, EG219, Eggplant Rootstock 302, and Fengshan No. 3, which were identified as highly resistant through inoculation with Ralstonia solanacearum, were propagated to serve as third-generation (G3) materials for eggplant rootstock disease resistance testing. These materials underwent continuous inoculation to observe the cumulative effects of resistance (Figure 1).
Regarding molecular marker analysis, PCR testing using SSR primers eme05B09 and emf11N23 (Khapte et al., 2018) cited in the literature revealed polymorphism. The PCR products were sequenced, and the resulting sequence information was aligned with the eggplant genome database. The results indicated that eme05B09 shared the highest similarity with sequence >Sme2.5_00446.1. Relevant fragments were downloaded to serve as templates for Single Nucleotide Polymorphism (SNP) discovery. Currently, six sets of PCR products have been selected for sequencing to facilitate further SNP exploration (Figure 2).
.jpg) ▲Figure 1. Comparison of disease susceptibility among materials was conducted using the average disease incidence rate on the 28th day following two rounds of inoculation. The eggplant variety "Yong-Zhuang" (F1 seeds) exhibited the highest resistance, followed by EG195-S3 and EG219-S3. Overall, the disease incidence rates of the third-generation (G3) materials were generally lower than those of the first-generation (G1) materials, which supports the hypothesis that disease resistance can be accumulated through successive generations. |
 ▲Figure 2. SNP exploration based on the relevant positions of primer emb05B09 in the literature. |
