This project collected appropriate germplasm and did intergeneric hybridization to create new orchid varieties, such as potted Vanda and mini Cattleya, which have a short juvenile period and improved low temperature tolerance. This year completed the collection of a total of 43 orchidvarieties, such as Holcoglossum spp., Rhychonopsis spp. and Cattyleaspp. and more than 250 intergenic hybridizations this year. Some seeds of mature pods have been planted, and 139 intergenic hybrids of seedlingshave grown and 25 varieties grew out of flask. Holcoglossum amesianum x Holcoglossum flavescens was bred and it only took two years from cross-pollination to flowering, which showed that the juvenile period is short. The plant is compact liked the male parent, more tolerant to low temperatures than its mother parent, its leaves grew at low temperatures of about 5-15°C and remain green. After long- day and cold treatment, H. pumilum is orchid flower induced by low temperature. In Cattleya, the results showed that genetic distance greatly affected the results of intergeneric hybridization. The breeding rate of intergeneric crosses within the Cattleya alliance is significantly higher than that of crosses between alliances in Laeliinae. Those with complex genetic backgrounds can be used as female parents to produce intergeneric offspring successfully. It is expected that excellent new strains can be selected from the hybrid combinations obtained by germination of the seed.

▲Figure 1. Holcoglossum amesianum x Holcoglossum flavescens, only takes two years from cross-pollination to vase planting and flowering. This shows its short juvenile period. The hybid plant is more tolerant to low temperatures than t Holcoglossum amesianum, the leaves grow at low temperatures of about 5-15°C and remain evergreen.

▲Figure 2. Holcoglossum pumilum was cultivated under low temperature and long-day in a growth chamber. It bloomed in September after 3 months cultivation. It is speculated that low temperature induced flowering in Holcoglossum pumilum.