2023 Research Project List
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Improvement on the Micropropagation Technique – In Vitro Propagation of Avocado (Persea americana Mill.) through Apical Buds and Nodal Segments

Topolin, an natural cytokinin,on micropropagation and adventitious shoot regeneration was evaluated on avocado ( Persea americana Mill. cv. Hass and Hall), Hass and Hall. In vitro shoots were grown for initiate the cultures on a multiplication medium containing 0.1 or 0.5 mg/L of Topolin and 1.0 mg/L kinetin. Regarding culture establishment the best results of shoot multiplication were achieved when 100 ml/L coconut water or 150 mg/L porato homogenate and 1.0 mg/L zeatin. were used as medium supplements.

Fig.1 Culture initiation of avocado (Persea americana Mill. cv.Hass) through nodal segments
Fig.1 Culture initiation of avocado (Persea americana Mill. cv.Hass) through nodal segments

 

Fig. 2. The effect of topolin concentration and organic supplements on shooting of nodal explants (avocado cv.Hass).

Fig.2. The effect of topolin concentration and organic supplements on shooting of nodal explants (avocado cv.Hass).