- Application of Herbal Medicine Plants in Animal Feed additives and establishment of GAP production system
- Study on the Development and Industrial Value of Biochar which made by used medium of Mushroom
- Establish the molecular detection procedures for quarantine pathogens on important vegetable seeds
- Establishment of single nucleotide polymorphism (SNP) markers for resistance genes detection in tomato and purity testing of F1 hybrid watermelon seed.
- Construction of Parameterized Management Wisdom Network for Vegetable Nursery
- Application of production management information system in plant tissue culture
- Study on the optimization harvest period and extraction of Vitis thunbergii Sieb. &Zucc. var.taiwaniana
- Development of functional Products for anti-aging and eye-protection from dendrobium, and Vitis thunbergii var.taiwaniana
- The Establishment of Next Generation Sequencing technique to detect potato virus
- Establishment of environmentally friendly seed production system for grain crops
- System optimization and popularization of plant seedling joint marketing information platform
- Establishment of Detection Techniques for Seedborne Pathogens of Tomato
- The establishment and application of specific molecular markers to detect bacterial wilt resistance gene in tomato.
- Determinant RNA detection of Bacterial spot of Tomato
- Development for multiple refined processing techniques to promoting seed quality
- Resistance breeding and industrial applications of anti-wilt for cucurbits and eggplant rootstock
- Establishment of highly effective isolated facility for healthy seedling
- Development of potyvirus resistance in melon using CRISPR/Cas9 technology
- The study of healthy seedling production by in vitro micropropagation
- The establishment and application of specific molecular markers for tomato bacterial spot resistance gene in tomato.
- Seed quality and seedling production technology research and development
- Construction of a certification system for plant seeds and seedlings─Establishment of molecular testing method for hybrid seed purity in pepper
- Study on Seed Vigor Detection Technology and Safe Storage Mode of Important Crops
- Intensification of Plant New Variety DUS Testing Technology and Research on Genetic Resources Management of the Potential Crop in Taiwan
- Export potential bulb flowers breeding and establishing the propagation system
- Breeding and Critical Techniques Development of Orchids.
- Breeding and evaluation of cucumber’s rootstock on water utilizing efficiency
- Study on producing cereal renewable pots and its effects on seedling production.
- Breeding for disease resistance in tomato and high quality eggplant.
- Breeding for Cucurbitaceae vegetables
- Breeding of high quality papaya varieties with international competitiveness
- Research on organic seed production of grain crops
- Establish a digital platform for seed illustration
- The performance evaluation and working tendency analysis for the seedling training courses in Farmers' Academy
- Enhancing industry connection of seed testing and international cooperation of ISTA
- 2018 Taiwan-Vietnam Plant Variety Protection Cooperation and Study on DUS Testing
- Study and Exchange on Plant Variety Protection System and DUS Testing Techniques between Taiwan and Indonesia
- Establishment of grafting technique and grafted seedling propagation system for oiltea camellia.
- Established qualitative detection method for unauthorized genetically modified orange petunias.
- Study of inspection acts for imported genetically modified feed and industrial applications traceability and export agricultural products
- Cacao breeding and development of vegetative propagation techniques
- Combine Tolerance of Papaya Ring Spot Viral Disease with All Hermaphroditic Character in Papaya Commercial Cultivar Adding Commercial Value.
- Establishment of foundation seed propagation system for potato variety‘Lunwei No.1’and‘Lunwei No.2’.
 Home > Achievement > 2018 Research Project List > Development of potyvirus resistance in melon using CRISPR/Cas9 technology |
Development of potyvirus resistance in melon using CRISPR/Cas9 technology
▲Fig.1. Protoplasts isolated from cotyledons of melon
▲Fig.2. Analysis of eIF4E gene polymorphism after PEG-mediated transformants.
Two candidate mutants were selected. (-: no restriction enzyme,BmgBI, digestion.
+: restriction enzyme digested)