Trichoderma sp. could inhibit the mycelium growth of Didymella bryoniae 70.97% on medium. In the trial of seed mixing with effective microorganism agent, Trichoderma sp. and Bacillus amyloliquefaciens showed better effect on rate of normal seedling 31% and 28%. On the control against D. bryoniae, the treatments of Trichoderma sp. and B.amyloliquefaciens were better with the lowest disease severity of 36% and 37%. In the wood vinegar trials, the results of normal seedling, abnormal seedling, hard seed, fresh seed and dead seed showed no difference, and the average germination rate on treated cucumber seeds was above 94%.However, the wood vinegar did not obviously inhibit mycelium growth of D. bryoniae.

▲Fig. Compare the ability of our Bacillus isolate (E)
with other effective microorganism against Didymella
bryoniae.  A is negative control.