The primary goal of this project is to enhance Taiwan's capacity for the monitoring and testing of agricultural biotechnological crops. We successfully established a Standard Operating Procedure for DNA quality assessment for quantitative genetically modified (GM) crop detection, summarizing technical guidance from EU documents to serve as a Quality Control basis. Method establishment included developing both qualitative and quantitative detection techniques for the GM maize event DP-915635-4, achieving a qualitative detection limit of 0.05%. Proficiency testing demonstrated high accuracy, with most participating laboratories achieving 100% correct results for maize and potato blind samples. However, one laboratory reported specific anomalies, including false-negative results for NPTII and PRSV-CP in papaya leaf samples, and a false-positive result for GTS40-3-2 in soybean powder. In the 2025 GM crop monitoring and testing program, a total of 171 samples were analyzed. These included 64 samples retrieved from domestic investigation fields (comprising 24 potatoes, 22 maize, and 18 soybeans), and 107 samples collected from imported crops (consisting of 69 soybeans, 36 maize, and 2 cottons).

▲Figure 1. Qualitative detection analysis results of GM maize event DP-915635-4.

▲Figure 2. Standard curve for quantitative analysis of genetically modified maize event (DP-915635-4).