The second-generation individual plants with high resistance confirmed by inoculation with bacterial wilt were collected for cultivation and seed production, and EG195, EG203, EG219, eggplant rootstock 302, Fengshan No. 3, KS2018 and Tainan No. 1 (S1) were collected as the third-generation materials for the disease resistance test of eggplant rootstock (Figure 1). The core seed of eggplant rootstock was stacked with original resistance, and the second-generation EG195 individual plant was used as the female parent, and EG203, EG219 eggplant rootstock 302, Fengshan No. 3, KS2018, Tainan No. 1 (F1) were used as pollen parents, and cross-pollination was performed to obtain a batch of hybrid seeds (Figure 2).

This batch of seeds will be included in the inoculation test material to verify whether there is a stacking effect of resistance.

The PCR results of the SSR primers eme05B09 and emf11N23 (Khapte et al., 2018) in the literature showed polymorphism. The PCR products were sequenced and the relevant sequence information was extracted and compared with the eggplant genome datebase platform (https://eggplant.kazusa.or.jp/index.html). The similarity between eme05B09 and >Sme2.5_00446.1 was the highest (Figure 3). The relevant fragments were downloaded as templates for SNP exploration and the generic primer design has been completed. The sequencing results of the PCR product of primer emf11N23 were limited, and no effective reference sequence could be obtained.

▲Figure 1. The second generation of individual plants with high resistance to bacterial wilt were collected for seed culture. EG195, EG203, EG219, eggplant rootstock 302, Fengshan No. 3, KS2018, and Tainan No. 1 (S1) were collected as the third generation eggplant rootstock materials.		▲Figure 2: Pollination is performed using EG195 as the female parent and high-resistance materials as the male parent to obtain more resistance stacking materials.
▲Figure 3. SNP exploration based on the relevant positions of primer emb05B09 in the literature.