Tomato soil-borne diseases may cause around 10-20% yield loss annually. And due to intensive cultivation and climate change, infectious cases are increasing. Recent studies have shown that adjusting root microbes may benefit the crops to become more resilient to environmental change. Therefore, this project aims to conduct whole-genomic sequencing of the cultivation soil from tomato root-knot nematode-resistant and susceptible genotype lines. This year we have collected the root-soil from field planting of tomato nematode-resistant and susceptible lines. The soil DNA extraction method has been well modified to obtain high-quality DNA from red clay. And the16S rRNA paired-end library has been constructed for next-generation sequencing. By analyzing the differences between the root microbiome, we wish to screen possible molecular targets and isolate the core microbes which could benefit the tomato growth.

▲Fig. 1. Plot design for tomato root-knot nematode-resistant and susceptible lines.

▲Fig. 2. DNA extracted from rhizosphere soil was checked in gel.