2021 Research Project List
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Research of optimization for detecting operation procedure on seed-borne pathogens

The goal of this program in 2021 was to establish an integrated operating procedure for detecting Pseudomonas spp. in order to improve efficiency and to decrease costs. Five specific primers were selected to detect P. syringae pv. tomato (Pst), P. viridiflava (Pv), P. cichorii (Pc), P. syringae pv. maculicola (Psm) and P. syringae pv. lachrymans (Pal), individually, and they were MM5F/MM5R (for Pst), 20F/20R (Pv), SfL1/SfR2 (for Pc), cflF/cflR (for Psm and Pst) and PalF/PalR (for Pal). The primers UpBacF/UpBacR for bacteria were used as internal control. Phosphate buffered saline (PBS) was better than 0.85 NaCl solution for extract bacteria from seeds. The standard operating procedure for detection has been drafted.

Phosphate buffered saline (PBS) was better than 0.85 NaCl solution for extract bacteria from seeds.▲Fig. 1. Phosphate buffered saline (PBS) was better than 0.85 NaCl solution for extract bacteria from seeds. Fig. 2. Five specific primers, MM5F/MM5R, 20F/20R, SfL1/SfR2, cflF/cflR and PalF/PalR, as well as one universal primers UpBacF/UpBacR (for bacteria) as internal control were used to detect Pseudomonas syringae pv. tomato (Pst), P. viridiflava (Pv), P. cichorii (Pc), P. syringae pv. maculicola (Psm) and P. syringae pv. lachrymans (Pal).
▲Fig. 2. Five specific primers, MM5F/MM5R, 20F/20R, SfL1/SfR2, cflF/cflR and PalF/PalR, as well as one universal primers UpBacF/UpBacR (for bacteria) as internal control were used to detect Pseudomonas syringae pv. tomato (Pst), P. viridiflava (Pv), P. cichorii (Pc), P. syringae pv. maculicola (Psm) and P. syringae pv. lachrymans (Pal).