2021 Research Project List
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Molecular Marker-Assisted Tomato Disease Resistance Breeding Selection Model Construction and Technology Development

Although Taiwan's tomato varieties are popular in the international market, as the varieties are continuously developed, the market life of the varieties has become shorter, and the pressure on variety research and development has become heavier.
The use of molecular markers for specific gene screening to confirm whether the contribution of the target gene is introduced into the reincarnation of the research and development strategy is widely used in the research and development of large seed companies; in contrast, Taiwanese seed companies are small in scale, and only a few have established molecular testing laboratories. With the ability to use molecular detection, other breeders still focus on field trait selection and urgently need assistance in introducing prospective selection techniques or providing assistance.

In this project, by cooperating with the industry, we will carry out the introduction and backcross breeding cooperation for tomato fusarium wilt resistance genes I-3. Designed SNP markers by collecting the tomato SNP database and genome sequence disclosed on the Internet. 188 sets of tomato background selection candidate marker groups have been developed; and the main prospect screening I-3 genotype analysis SCAR marker 2 sets have been selected through the context selection to accelerate the efficiency of backcrossing, shorten the selection period, and promote the operation model to the industry.

The CAPS-SR5 marker (Catanzariti et al., 2015) is a CAPs-type molecular marker developed for the SpSRLK-5 gene. In order to reduce the cost of screening and simplify the screening process, this study improved the CAPS-SR5 marker. Convert CAPs to codominant SCAR markers. SCAR markers I-3-SCAR#28 and I-3-SCAR#29 were verified by PCR and showed that they were consistent with the results of CAP-SR5.
Fig. 1. The CAPS-SR5 marker (Catanzariti et al., 2015) is a CAPs-type molecular marker developed for the SpSRLK-5 gene. In order to reduce the cost of screening and simplify the screening process, this study improved the CAPS-SR5 marker. Convert CAPs to codominant SCAR markers. SCAR markers I-3-SCAR#28 and I-3-SCAR#29 were verified by PCR and showed that they were consistent with the results of CAP-SR5.