In this study, the statistical model of method validation for the qualitative data has been established. We built the analysis manual and source code and demonstrated in a learning course. The real data from the literature were analyzed to illustrate the procedure of method validation. Appropriate sampling amount for the determination of crop seed moisture content shows that the standard group and the halved group of rice, peanuts and watermelon have the same representativeness, and the halved group does not have a risk of insufficient seed quantity to complete the moisture content determination. Therefore, the three crops can consider halving the sample size for inspection. The germination obtained in a laboratory does not often match with field emergence, and both tests are time consuming. The current study was aimed to establish vigor test methods for predicting field emergence in tomato. The methods included accelerated aging method, radical emergence method, TTC dying method and electrical conductivity method were tested against field emergence and laboratory germination at 20/30℃. The correlation coefficient between FGP_F and FGP_20/30 or FGP_AA enable to reflect emergency but the test consuming was 14 and 17 days, respectively. For raising the examination efficiency, radical emergence at single sample time was suggested. The results of sorghum seeds showed a significant positive correlation between G_S^4th and GF_S in the standard germination test, GF^4th and GF_F in the field unearthed test, RE₆₄ and TTC in the radicle protrusion test, and a significant negative correlation between the EC value and RE₃₂. Fast green There is no significant correlation with any experimental treatment.

▲Fig. 1.The correlation analysis between accelerated aging method, radical emergence method, TTC dying method and electrical conductivity method in tomato seed.

▲Fig. 2. The correlation analysis between seed vigor treatments of sorghum