- Establishment of friendly cultivation module for cocoa seedlings
- Analysis of the arrangement of Nectar Plants under the Climate Change.
- Establishment of resilience cultivation practices by increase drought-tolerant of potato and ensure the production of leafy vegetables after typhoon destructed and rain disaster
- Research on Improving Agricultural Workers' Adaptation Strategies in Response to Climate Change: A Case Study of Agricultural Workers in Seedling production industries.
- Research on processing technology for reducing the waste of domestic agricultural production
- Establishment of techniques to reduce waste and create valueadded utilization of tomato seed processing by-producet and seasonal overproduced tomato.
- Establish the system of value-added utilization on pumpkin residues from seedling industry
- Development of value-added products from seeded papaya wastes
- Developement of eco-friendly nursery material technology
- Application of Herbal Medicine Plants in Animal Feed Additives and Establishment of GAP Production System.
- Construction and promotion of organic demonstration nursery
- Study on the development and industrial value of biochar which made by used media of mushroom
- Development of disinfection technique for important seed-borne pathogens of vegetables
- Research and application of value-added and waste reduction technology in green supply chain
- Construction of Intelligent Production and Sale Management Network for Vegetable Nursery
- Establishment of physiological parameters and production operation mode for vegetable nursery
- Modular development and application of production management information system in plant tissue culture
- The establishment of high through-put sequencing workflow to detect pathogens of tomato seeds
- Establishment of organic seed production and supplying system for grain crops
- Using seed priming treatment to promote crop stress tolerance and survey industry status
- Establishment of highly effective isolated facility for healthy seedling
- Development and application of molecular markers for the important traits of Solanaceae crops
- Development of potyvirus resistance in melon using CRISPR/Cas9 technology
- The Study of Healthy Seedling Production by In-vitro Micropropagation
- Research on optimization of detecting operation procedure for seed-borne pathogens
- Study and improvement of strengthening seed testing technology
- Strengthen Plant New Variety DUS Testing Techniques and Research on Genetic Resources Management and Application of the Potential Crops in Taiwan
- Breeding and key techniques research on orchids and bulb flowers
- Breed and assess the drought tolerance rootstocks with cucumber
- Breeding for disease resistant tomato and superior quality eggplant.
- Breeding for environment tolerance and high quality of Cucurbitaceae vegetables.
- Breeding of high quality papaya varieties with international competitiveness
- Establishment of environmentally friendly seed production system for grain crops
- Establish a digital platform for seed illustration
- Establishment of automatic seedling evaluation system and integration of seed testing platform
- Intelligent Marketing and Promotion of Seedling Production and Marketing Information Management System.
- Establishment of Phalaenopsis Varieties Identification and Application Integration Platform
- Research on the Integrated Planning and working tendency analysis for the seedling training courses in Farmers' Academy.
- Harmonization and Cooperation on DUS testing between Taiwan and Japan
- Taiwan-Israel Plant Variety Protection Cooperation and Study on DUS Testing
- Enhancing the seed testing technical cooperation with ISTA and new southbound countries
- Establishment of inspection and monitoring system for new era genetically modified organisms
- Study of inspection acts for imported genetically modified feed and industrial applications traceability and export agricultural products
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Development of potyvirus resistance in melon using CRISPR/Cas9 technology
▲Fig.1. sgRNA targeted mutation detected in melon plants induced by CRISPR/Cas9 vector.
▲Fig. 2.RT-PCR detection after mutants were inoculated with ZYMV for 7days.
M:100bp ladders; 1: H2O; 2-3: Zucchini+ZYMV；4-6: melon control；7-11: melon control +ZYMV；12-16: M251+ZYMV；17-20: M252+ZYMV