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Development and application of molecular markers for the important traits of Solanaceae crops

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Fig. 1. Tomato bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum Smith. Although the infected plants remain green, they wither and die quickly. A cross section of an infected stem shows a brown discoloration of the vascular tissue and milky sticky bacteria mud exudates.
▲Fig. 1. Tomato bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum Smith. Although the infected plants remain green, they wither and die quickly. A cross section of an infected stem shows a brown discoloration of the vascular tissue and milky sticky bacteria mud exudates.

Fig 2. Shin et al. (2020) conducted a QTL resistance locus study on Hawaii7996, which has stable resistance to tomato bacterial wilt. The results showed that there are four QTLs on chromosome 6 and three QTLs on chromosome 12. The resistance of QTLs on chromosome 6 to tomato bacterial wilt pathogen is not as good as the QTLs on chromosome 12, but it has wider resistance and can tolerate endemic strains in different regions.
▲Fig 2. Shin et al. (2020) conducted a QTL resistance locus study on Hawaii7996, which has stable resistance to tomato bacterial wilt. The results showed that there are four QTLs on chromosome 6 and three QTLs on chromosome 12. The resistance of QTLs on chromosome 6 to tomato bacterial wilt pathogen is not as good as the QTLs on chromosome 12, but it has wider resistance and can tolerate endemic strains in different regions.

Fig 3. The marker Bwr6-10 developed for the resistance gene of tomato bacterial wilt on chromosome 6 is polymorphic in resistance to susceptible materials, increasing by 734bp and 534bp bands respectively, which is clearer and easier to interpret than the literature.
▲Fig 3. The marker Bwr6-10 developed for the resistance gene of tomato bacterial wilt on chromosome 6 is polymorphic in resistance to susceptible materials, increasing by 734bp and 534bp bands respectively, which is clearer and easier to interpret than the literature.

(b.) Development of the molecular marker for male sterility and fertility restorer in Capsicum Annuum L.

Fig 1. The genotyping result of real-time PCR of KASP assay for detecting fertility restorer (Rf) gene in pepper. The CaChr6-2 (right) developed in this study has a better cluster result than the reference SNP site, ColMod1 (left).
▲Fig 1. Fig 1. The genotyping result of real-time PCR of KASP assay for detecting fertility restorer (Rf) gene in pepper. The CaChr6-2 (right) developed in this study has a better cluster result than the reference SNP site, ColMod1 (left).