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Establishment of inspection and monitoring system for new era genetically modified organisms

On June 7, 2019, genetically engineered (GE) wheat plants were detected on the field in Washington State. After investigated, the GE wheat plants in question were identified as MON 71300 and MON 71800 developed by Monsanto (now owned by Bayer CropScience). Because these two lines were not commercial sold on market, we constructed reference plasmid DNA by gene synthesis method.
▲Fig.1. On June 7, 2019, genetically engineered (GE) wheat plants were detected on the field in Washington State. After investigated, the GE wheat plants in question were identified as MON 71300 and MON 71800 developed by Monsanto (now owned by Bayer CropScience). Because these two lines were not commercial sold on market, we constructed reference plasmid DNA by gene synthesis method.

The flowchart of GM wheat MON71800/71300 detection. Element-specific CP4-EPSPS and event-specific MON71800 primers were used for GM wheat detection and wheat AccI gen was used for internal control. We expect this method could distinguish GM wheat MON71800 and MON71300 event, respectively.
▲Fig. 2. The flowchart of GM wheat MON71800/71300 detection. Element-specific CP4-EPSPS and event-specific MON71800 primers were used for GM wheat detection and wheat AccI gen was used for internal control. We expect this method could distinguish GM wheat MON71800 and MON71300 event, respectively.