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Research of optimization for detecting operation procedure on seed-borne pathogens and development of seed disinfection technique

Pseudomonas spp. are important bacterial plant pathogens and are valued in international seed trade market owing to their seed transmission. The goal of this study was to establish an integrated operating procedure for detecting five Pseudomonas spp., including P. syringae pv. tomato (Pst), P. viridiflava (Pv), P. cichorii (Pc), P. syringae pv. maculicola (Psm) and P. syringae pv. lachrymans (Pal) on Solanaceae seeds in order to improve efficiency and to decrease costs. Phosphate buffered saline (PBS) was first used for extract bacteria from seeds. Then, suspected bacteria were isolated by using semi-selective media.  Finally, five specific primers, including MM5F/MM5R (for Pst), 20F/20R (Pv), SfL1/SfR2 (for Pc), cflF/cflR (for Psm and Pst) and PalF/PalR (for Pal), were adopted to identify the Pseudomonas spp. individually.

Fig.1