Grape cultivation generally uses cutting or grafting methods. If the mother plant carries a virus, its propagated offspring will be virus-infected seedlings that leading to weakened plants and affecting fruit quality and yield. In recent years, TSIPS has employed tissue culture technology to produce virus-free, healthy seedlings. During this process, unusual symptoms were observed. This research project aims to collect diseased materials from various production areas, conduct nucleic acids extraction for small RNA next-generation sequencing, to establish a grape virus detection platform, and gain insights into virus infections in domestic grape plants. In the first half of the year, we collected 50 samples from five varieties in key production areas such as Xinshe and Dacun. Following nucleic acid extraction, PCR was performed using grape plant 18S rDNA primers to evaluate the effectiveness of the extraction process. Additionally, ELISA testing was conducted on the collected materials using five grape virus (GVA, GFLV, GLRaV-1, GLRaV-3, and GFkV) serological diagnostic methods previously established by our laboratory to gain a preliminary understanding of virus infections. The results showed that out of 28 samples tested from Xinshe, 6 samples were virus-positive: 2 were solely infected with GFkV, 3 had mixed infections (1 with GVA+GFkV, 2 with GLRaV-3+GFkV), and 1 with a triple virus infection (GVA+GLRaV-3+GFkV). Out of 22 samples tested from Dacun, 5 samples were virus-positive: 3 were solely infected (1 with GLRaV-3, 2 with GFkV), 1 had a mixed infection of GLRaV-3+GFkV, and 1 had a triple virus infection (GLRaV-1+GLRaV-3+GFkV).

▲Fig. 1 The diseased materials collected from Xinshe and Dacun areas were tested using ELISA with five grape virus serological methods.		▲Fig. 2 Leaf curl symptoms in Xinshe grapevines (Kyoho).
▲Fig. 3 Herbicide damage symptoms in Dacun grapevines (Kyoho).