Healthy and high-quality seedlings are the foundation of the industry, and only varieties with good traits can meet industry needs. This project uses tissue culture technology to establish tools for assisted selection, accelerating the breeding process. Conditions for anther culture or microspore culture have been successfully established for various Brassicaceae crop varieties. Anther culture conditions for broccoli (Brassica oleracea) and Chinese kale (Brassica alboglabra) were established, with the culture conducted on C1 and C3 media, achieving a differentiation rate of approximately 6.7-20%. Regarding the conservation and quantification of green bamboo germplasm, as well as the flowering induction of tissue-cultured plants, a subculture frequency of once every two weeks led to the survival of shoot tissues. The addition of 0.01 mg/L NAA, 1 mg/L BA, and 0.1 mg/L TDZ to the MS basal medium resulted in better shoot proliferation rates. For the flowering induction of tissue-cultured green bamboo plants after acclimatization, plants cultured on TDZ 0.1 showed the smallest plant height, leaf length, and leaf width. In the initial proliferation phase in vitro, they exhibited the highest proliferation rate, but no significant differences were observed in subsequent subcultures compared to other media.

▲Fig.1. Growth of different strains of green bamboo after 7 generations of continuous propagation through tissue culture, A. Dapang, B. Hongyu.		▲Fig.2. Growth performance of green bamboo seedlings cultured in medium supplemented with different cytokinins for flowering induction after 2 months of cultivation
▲Fig.3. The growth performance of green bamboo tissue culture seedlings that were cultured in medium supplemented with different cytokinins for flowering induction after 2 months of cultivation. From left to right: BA3, BA4, TDZ0.1, TDZ0.5.		▲Fig.4. Changes in the proliferation rate of shoots of green bamboo tissue culture seedlings in continuous culture in medium containing different cytokinin types and concentrations.
▲Fig.5. Differentiation ratio of cruciferous anther culture using medium with different salt formulations.