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Tomato Multiple pathogen detection chip development by TSIPS
To reduce pathogens test time and cost
Taiwan Seed Improvement and Propagation station (TSIPS), C.O.A. refer that the technology of "primer pairs and primer probe sets for Tomato virus nucleic acid detection chips" was transferred to industry applications successfully in 2019. Due to the rising demand for tomato seed in global market, the quarantine requirements for tomato seed pathogens are also increasing. Using Tomato pathogen detection chip to detect pathogen within tomato seeds and seedling, 76 percent test time and 45 percent cost are reduced.
TSIPS, C.O.A. indicates that according to 2019 Taiwan Agricultural statistics yearbook, the annual export value of Taiwan tomato seeds reached NTD 510 million. The global tomato seed market was valued at is projected to register a CAGR of 7.9% over the forecast period (2020-2025). The tomato seeds are produced and traded across the globe and can carry and spread diseases and pest. Various countries have increasingly higher quarantine requirements for tomato seed-borne pathogens detection. TSIPS, C.O.A. refer that the technology of "primer pairs and primer probe sets for solanaceous crop virus nucleic acid detection chips" was transferred to industry applications under non-exclusive authorization in 2019. The important pathogens targets include Potato spindle tuber viroid (PSTVd), Tomato chlorotic dwarf viroid (TCDVd), Tomato apical stunt viroid(TASVd), Pepper chat fruit viroid (PCFVd), Columnea latent viroid (CLVd), Tomato planta macho viroid(TPMVd). Under this non-exclusive authorization, company increases the pathogen detection targets in their own biochips. The seed sample pretreatment technology have developed to reduce the nursery time from 21 days inspection period to 5 days, which greatly shortens the inspection period by 76%. More than 11different pathogens could be test in one single reaction can detect, which can reduce the test cost by 45% and expand the benefits of technology research and development.
TSIPS, C.O.A. indicates that the United Nations has declared 2020 as the International Year of Plant Health (IYPH). The year is an opportunity to raise global awareness on how protecting plant health can help end hunger, reduce poverty, protect the environment, and boost economic development. Healthy seedlings (seeds) are the primary requirements for plant health management actions. TSIPS, C.O.A. will continue to develop pathogen detection technology, provide Taiwan seed health inspection services, and enhance the international competitiveness of Taiwan seed industries.
▲Fig. 1 Tomato Diseases were detected by tomato pathogen detection chips developed by government and business.